Hotspot

rpl20-rps12

LSC · 72630-73345 · 716 bp

Back to Brocchinia

Pi 0.00903

MarkerSeek score 64.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 16
pi: 0.00903
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AGTCTTGAATGAGCCCCTCT	TCTCCCGCTCTTAGAGGATG	50.0	55.0	58.0	58.0	860-867 bp	1.0	81.3
rpl20-rps12_p2	TGAGCCCCTCTAAAGGTTGA	TCTCCCGCTCTTAGAGGATG	50.0	55.0	58.0	58.0	851-858 bp	1.0	81.3
rpl20-rps12_p3	TTTTTGTTCGACGTCTCCGA	TCTCCCGCTCTTAGAGGATG	45.0	55.0	57.8	58.0	817-824 bp	1.0	80.0
rpl20-rps12_p4	TTTTGTTCGACGTCTCCGAG	TCTCCCGCTCTTAGAGGATG	50.0	55.0	58.2	58.0	816-823 bp	1.0	79.8
rpl20-rps12_p5	AGTCTTGAATGAGCCCCTCT	TGTTACGAAATCTCCCGCTC	50.0	50.0	58.0	57.7	870-877 bp	1.0	79.1