Hotspot

ycf4-cemA

LSC · 63253-64073 · 821 bp

Back to Brocchinia

Pi 0.0209

MarkerSeek score 63.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 42
pi: 0.0209
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	TGGGAAAGAAGGCAATGCTT	45.0	45.0	57.9	58.0	880-905 bp	1.0	84.6
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	TGGGAAAGAAGGCAATGCTT	50.0	45.0	58.1	58.0	882-907 bp	1.0	84.3
ycf4-cemA_p3	TTTCGTTGGGGATTTCCTGG	TGGGAAAGAAGGCAATGCTT	50.0	45.0	58.1	58.0	1097-1122 bp	1.0	83.8
ycf4-cemA_p4	GGGGGTCTCGAAAAACAAGT	TGGGAAAGAAGGCAATGCTT	50.0	45.0	58.0	58.0	1369-1394 bp	1.0	83.7
ycf4-cemA_p5	GGGGGTCTCGAAAAACAAGT	TATGTGTCTCCGGGTACTCC	50.0	55.0	58.0	57.9	1577-1602 bp	0.833333	75.7