Hotspot

accD-psaI

LSC · 61417-62231 · 815 bp

Back to Brocchinia

Pi 0.0254

MarkerSeek score 59.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 47
pi: 0.0254
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGTACCCGATGGTTCAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1036-1277 bp	1.0	84.5
accD-psaI_p2	GACAGTACCCGATGGTTCAC	ACTTGTTTTTCGAGACCCCC	55.0	50.0	58.0	58.0	1492-1727 bp	1.0	83.9
accD-psaI_p3	AGACAGTACCCGATGGTTCA	ACTTGTTTTTCGAGACCCCC	50.0	50.0	58.1	58.0	1493-1728 bp	1.0	83.4
accD-psaI_p4	GGTTTCTACGGACCCCATTC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1528-1769 bp	1.0	83.1
accD-psaI_p5	GGTTTCTACGGACCCCATTC	ACTTGTTTTTCGAGACCCCC	55.0	50.0	58.0	58.0	1984-2219 bp	1.0	82.9