Hotspot

rbcL-accD

LSC · 59179-59949 · 771 bp

Back to Brocchinia

Pi 0.0219

MarkerSeek score 66.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 35
pi: 0.0219
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	TTCTTCACCTCCACCGGTAT	55.0	50.0	58.0	58.1	970-986 bp	0.333333	55.1
rbcL-accD_p2	TGCCTGCTCTTACCGAAATC	TTCTTCACCTCCACCGGTAT	50.0	50.0	58.0	58.1	1153-1169 bp	0.333333	54.8
rbcL-accD_p3	TGATCTTGCTCGTGAAGGTG	TTCTTCACCTCCACCGGTAT	50.0	50.0	57.9	58.1	1008-1024 bp	0.333333	54.6
rbcL-accD_p4	AATGCTACTGCGGGTACATG	TTCTTCACCTCCACCGGTAT	50.0	50.0	58.0	58.1	1592-1608 bp	0.333333	54.1
rbcL-accD_p5	GTTATACCTGTGGCTTCCGG	TTCTTCACCTCCACCGGTAT	55.0	50.0	58.1	58.1	1209 bp	0.166667	45.8