Hotspot

atpB-rbcL

LSC · 56923-57717 · 795 bp

Back to Brocchinia

Pi 0.0179

MarkerSeek score 61.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 30
pi: 0.0179
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	921-933 bp	1.0	82.5
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	899-911 bp	1.0	82.3
atpB-rbcL_p3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	58.1	58.4	922-934 bp	1.0	80.1
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	900-912 bp	1.0	79.9
atpB-rbcL_p5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.5	58.1	900-912 bp	1.0	79.4