Hotspot

trnT-UGU-trnL-UAA

LSC · 48649-49461 · 813 bp

Back to Brocchinia

Pi 0.0267

MarkerSeek score 63.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 43
pi: 0.0267
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	560-931 bp	1.0	87.3
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	55.0	50.0	58.2	58.0	591-962 bp	1.0	86.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	568-939 bp	1.0	86.8
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	55.0	50.0	58.2	58.1	599-970 bp	1.0	86.4
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	583-954 bp	1.0	85.4