Hotspot

psaA-ycf3

LSC · 43994-44632 · 639 bp

Back to Brocchinia

Pi 0.0171

MarkerSeek score 65.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 26
pi: 0.0171
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GTTGAAAATCACGAAGCGCT	50.0	45.0	57.9	57.7	743-746 bp	1.0	79.8
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	787-790 bp	1.0	79.3
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	CCGATCAAGCTGCTGAGTAT	50.0	50.0	57.9	57.5	816-819 bp	1.0	77.9
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TCCGATCAAGCTGCTGAGTA	50.0	50.0	57.9	58.5	817-820 bp	1.0	77.9
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAAATCACGAAGCGCTTC	50.0	45.0	57.9	57.4	741-744 bp	1.0	77.6