Hotspot

psbC-trnS-UGA

LSC · 37508-37644 · 137 bp

Back to Brocchinia

Pi 0.0384

MarkerSeek score 64.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 11
pi: 0.0384
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	244-260 bp	1.0	81.8
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	283-299 bp	1.0	81.5
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	255-271 bp	1.0	81.1
psbC-trnS-UGA_p4	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	229-245 bp	1.0	81.0
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	294-310 bp	1.0	80.9