Hotspot

trnE-UUC-trnT-GGU

LSC · 33707-33978 · 272 bp

Back to Brocchinia

Pi 0.0445

MarkerSeek score 71.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 272
variable_sites: 33
pi: 0.0445
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	354-845 bp	1.0	84.3
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	375-866 bp	1.0	84.2
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	333-824 bp	1.0	84.2
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	354-845 bp	1.0	84.0
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	351-842 bp	1.0	83.9