Hotspot

trnK-UUU-rps16

LSC · 4069-4780 · 712 bp

Back to Brocchinia

Pi 0.0238

MarkerSeek score 56.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 37
pi: 0.0238
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGAGGATCAGTCGTGGTCTT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1488-1495 bp	1.0	82.5
trnK-UUU-rps16_p2	TGAGGATCAGTCGTGGTCTT	GATCCCCGGGGTAATGTCTA	50.0	55.0	58.1	58.0	1452-1455 bp	0.833333	75.0
trnK-UUU-rps16_p3	TGAGGATCAGTCGTGGTCTT	GAATAAGGATCCCCGGGGTA	50.0	55.0	58.1	58.0	1459-1462 bp	0.833333	74.9
trnK-UUU-rps16_p4	TCACAGGAGAACCCACTCTT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	57.9	58.0	1852-1856 bp	0.833333	74.9
trnK-UUU-rps16_p5	TCACAGGAGAACCCACTCTT	GATCCCCGGGGTAATGTCTA	50.0	55.0	57.9	58.0	1816-1819 bp	0.833333	74.9