Hotspot

ndhF

SSC · 102001-104220 · 2220 bp

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Pi 0.0189
MarkerSeek score 53.50
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
42
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AAAATCGCGGAAAACCGAAC ACGAAACAAGAGAGCTGCTT 45.0 45.0 58.0 57.8 2693-2697 bp 1.0 79.9
ndhF_p2 AACACCCATCAATGCATGGT ACGAAACAAGAGAGCTGCTT 45.0 45.0 58.1 57.8 2797-2801 bp 1.0 79.8
ndhF_p3 ACCCATCAATGCATGGTGTT ACGAAACAAGAGAGCTGCTT 45.0 45.0 58.1 57.8 2794-2798 bp 1.0 79.8
ndhF_p4 AATCGCGGAAAACCGAACTA ACGAAACAAGAGAGCTGCTT 45.0 45.0 57.9 57.8 2691-2695 bp 1.0 79.6
ndhF_p5 TCGCGGAAAACCGAACTATT ACGAAACAAGAGAGCTGCTT 45.0 45.0 57.9 57.8 2689-2693 bp 1.0 79.6