Hotspot

rpl32-trnL-UAG

SSC · 105304-105950 · 647 bp

Back to Briza

Pi 0.0376
MarkerSeek score 76.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
647
variable_sites
23
pi
0.0376
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GTTTTTCGGGGCAACAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.0 58.0 721-745 bp 1.0 86.2
rpl32-trnL-UAG_p2 TTTTCGGGGCAACAACAAAC TTCGAACCGAGATGCTTGAG 45.0 50.0 58.0 58.0 719-743 bp 1.0 86.2
rpl32-trnL-UAG_p3 TTTTCGGGGCAACAACAAAC GGATTCGAACCGAGATGCTT 45.0 50.0 58.0 58.1 722-746 bp 1.0 85.8
rpl32-trnL-UAG_p4 GTTTTTCGGGGCAACAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 58.0 58.1 724-748 bp 1.0 85.7
rpl32-trnL-UAG_p5 GGGGCAACAACAAACAAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 57.9 58.0 714-738 bp 1.0 85.6