Hotspot

ndhH-ndhF

SSC · 101968-102000 · 33 bp

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Pi 0.0909
MarkerSeek score 71.60
Primer available yes
Label ndhH-ndhF

Feature Metadata

label_name
ndhH-ndhF
feature_type
igs
region
SSC
length_bp
33
variable_sites
3
pi
0.0909
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-ndhF_p1 AAAATCGCGGAAAACCGAAC CGGATAATGGAATAGCGGGG 45.0 55.0 58.0 58.0 639-644 bp 1.0 83.1
ndhH-ndhF_p2 TCGCGGAAAACCGAACTATT CGGATAATGGAATAGCGGGG 45.0 55.0 57.9 58.0 635-640 bp 1.0 82.8
ndhH-ndhF_p3 AACACCCATCAATGCATGGT CGGATAATGGAATAGCGGGG 45.0 55.0 58.1 58.0 743-748 bp 1.0 82.7
ndhH-ndhF_p4 ACCCATCAATGCATGGTGTT CGGATAATGGAATAGCGGGG 45.0 55.0 58.1 58.0 740-745 bp 1.0 82.7
ndhH-ndhF_p5 CTTTTGGGAAAACGTCGACG CGGATAATGGAATAGCGGGG 50.0 55.0 58.0 58.0 1031-1035 bp 1.0 82.6