Hotspot

psbE-petL

LSC · 62087-63242 · 1156 bp

Back to Briza

Pi 0.0247
MarkerSeek score 55.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1156
variable_sites
12
pi
0.0247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.42
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA GCCAATAAATAGAGCTGGGGT 45.0 47.6 58.0 57.8 692-1343 bp 1.0 75.0
psbE-petL_p2 CAACCCGCAATGAATAGGGA GCCAATAAATAGAGCTGGGGT 50.0 47.6 57.9 57.8 681-1332 bp 1.0 74.7
psbE-petL_p3 ACCAACCCGCAATGAATAGG GCCAATAAATAGAGCTGGGGT 50.0 47.6 58.2 57.8 683-1334 bp 1.0 73.2
psbE-petL_p4 TGACAAATAACCAACCCGCA AGCCAATAAATAGAGCTGGGG 45.0 47.6 58.0 57.5 693-1344 bp 1.0 72.7
psbE-petL_p5 CAACCCGCAATGAATAGGGA AGCCAATAAATAGAGCTGGGG 50.0 47.6 57.9 57.5 682-1333 bp 1.0 72.4