Hotspot

ycf3-trnS-GGA

LSC · 43685-44295 · 611 bp

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Pi 0.0313
MarkerSeek score 71.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
611
variable_sites
19
pi
0.0313
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.1 58.2 794-796 bp 1.0 80.6
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTCAA 50.0 45.0 58.1 57.8 744-746 bp 1.0 80.4
ycf3-trnS-GGA_p3 TCTCCTGAGGTTGTCGGAAT AGTTCCAATGCTACGCCTTC 50.0 50.0 58.1 58.3 746-748 bp 1.0 79.9
ycf3-trnS-GGA_p4 TTCTCCTGAGGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.3 58.2 795-797 bp 1.0 78.8
ycf3-trnS-GGA_p5 TTTCTCCTGAGGTTGTCGGA ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.3 58.2 796-798 bp 1.0 78.8