Hotspot

petN-trnC-GCA

LSC · 17240-18152 · 913 bp

Back to Briza

Pi 0.0303
MarkerSeek score 69.80
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
913
variable_sites
27
pi
0.0303
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 948-962 bp 1.0 74.6
petN-trnC-GCA_p2 AGCCCAAGCGAGACTTACTA CCAGTTCAAATCTGGGTGCC 50.0 55.0 58.2 59.1 950-964 bp 1.0 74.1
petN-trnC-GCA_p3 CCACTCCTCCCCCATACTAC AGTTCAAATCTGGGTGCCG 60.0 52.6 58.3 58.1 1004-1018 bp 1.0 73.3
petN-trnC-GCA_p4 CCACTCCTCCCCCATACTAC CCAGTTCAAATCTGGGTGCC 60.0 55.0 58.3 59.1 1006-1020 bp 1.0 72.8
petN-trnC-GCA_p5 AGCCCAAGCGAGACTTACTA CAGTTCAAATCTGGGTGCCG 50.0 55.0 58.2 59.5 949-963 bp 1.0 71.2