Hotspot

trnD-GUC-psbM

LSC · 16047-16755 · 709 bp

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Pi 0.0275
MarkerSeek score 70.00
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
709
variable_sites
19
pi
0.0275
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG AGAATGAACAGTGCAGTAGC 50.0 45.0 58.5 55.8 807-820 bp 1.0 62.0
trnD-GUC-psbM_p2 GTTCAATTGGTCAGAGCACC AGAATGAACAGTGCAGTAGC 50.0 45.0 57.0 55.8 808-821 bp 1.0 57.8
trnD-GUC-psbM_p3 CGCGGGGATTGTAGTTCAA AGAATGAACAGTGCAGTAGC 52.6 45.0 57.9 55.8 821-834 bp 1.0 56.6
trnD-GUC-psbM_p4 GGTATGCCATACATCCCGC AGAATGAACAGTGCAGTAGC 57.9 45.0 58.1 55.8 837-850 bp 1.0 56.5
trnD-GUC-psbM_p5 TCAATTGGTCAGAGCACCG AGAATGAACAGTGCAGTAGC 52.6 45.0 57.8 55.8 806-819 bp 1.0 56.0