Hotspot

trnfM-CAU-trnG-UCC

LSC · 12748-12853 · 106 bp

Back to Briza

Pi 0.0294
MarkerSeek score 69.90
Primer available yes
Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name
trnfM-CAU-trnG-UCC
feature_type
igs
region
LSC
length_bp
106
variable_sites
3
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-UCC_p1 CCTCAAGGTTATGAGCCTCG AGCCTTCCAAGCTAACGATG 55.0 50.0 57.8 58.0 197-198 bp 1.0 80.7
trnfM-CAU-trnG-UCC_p2 CCTCAAGGTTATGAGCCTCG CCTAGCCTTCCAAGCTAACG 55.0 55.0 57.8 58.1 200-201 bp 1.0 80.5
trnfM-CAU-trnG-UCC_p3 CCTCGTGAGCTACCAAACTG AGCCTTCCAAGCTAACGATG 55.0 50.0 58.3 58.0 182-183 bp 1.0 80.5
trnfM-CAU-trnG-UCC_p4 CCTCGTGAGCTACCAAACTG CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.3 58.1 185-186 bp 1.0 80.3
trnfM-CAU-trnG-UCC_p5 CCTCAAGGTTATGAGCCTCG CCAACGTCGACTATAACCCC 55.0 55.0 57.8 57.8 218-219 bp 1.0 79.0