Hotspot

trnS-GCU-psbD

LSC · 7737-8646 · 910 bp

Back to Briza

Pi 0.0352
MarkerSeek score 71.80
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
LSC
length_bp
910
variable_sites
32
pi
0.0352
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 45.0 45.0 58.0 58.2 1042-1106 bp 1.0 80.3
trnS-GCU-psbD_p2 ATTAGCAATCCGCCGCTTTA CCCTTCGTAACCAGTCATCC 45.0 55.0 58.0 57.7 1026-1090 bp 1.0 79.8
trnS-GCU-psbD_p3 GAGCGGAAAGAGAGGGATTC ACAAAAACGAAACGGTCCCT 55.0 45.0 57.8 58.2 1093-1157 bp 1.0 78.6
trnS-GCU-psbD_p4 GAGCGGAAAGAGAGGGATTC CCCTTCGTAACCAGTCATCC 55.0 55.0 57.8 57.7 1077-1141 bp 1.0 78.1
trnS-GCU-psbD_p5 ATTAGCAATCCGCCGCTTTA GAAACGGTCCCTTCGTAACC 45.0 55.0 58.0 58.6 1034-1098 bp 1.0 77.7