Hotspot

ycf1

SSC · 124019-129700 · 5682 bp

Back to Breynia

Pi 0.0077

MarkerSeek score 43.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5682
variable_sites: 83
pi: 0.0077
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGGAATATTAATGATACCTAACA	CTGATCATTCGCAGAAACACA	29.2	42.9	53.2	56.9	259-265 bp	1.0	44.3
ycf1_p2	TCGGAATATTAATGATACCTAACA	TGATCATTCGCAGAAACACA	29.2	40.0	53.2	55.7	258-264 bp	1.0	44.3
ycf1_p3	TCGGAATATTAATGATACCTAACAA	CTGATCATTCGCAGAAACACA	28.0	42.9	53.9	56.9	259-265 bp	1.0	44.3
ycf1_p4	TTCGGAATATTAATGATACCTAACA	CTGATCATTCGCAGAAACACA	28.0	42.9	53.9	56.9	260-266 bp	1.0	44.2
ycf1_p5	TCGGAATATTAATGATACCTAACA	TCTGATCATTCGCAGAAACACA	29.2	40.9	53.2	58.3	260-266 bp	1.0	44.2