Hotspot

ndhF

SSC · 110120-112354 · 2235 bp

Back to Breynia

Pi 0.00589

MarkerSeek score 37.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 25
pi: 0.00589
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCGGAACTTCTTCGTAGTG	AGAGCGGATGAATCAGAAACA	55.0	42.9	58.0	57.4	2776-2794 bp	1.0	69.0
ndhF_p2	GAGCTACTGAGGAACAACGG	AGAGCGGATGAATCAGAAACA	55.0	42.9	58.0	57.4	2871-2889 bp	1.0	68.9
ndhF_p3	GAGGAACAACGGGAGCTTAG	AGAGCGGATGAATCAGAAACA	55.0	42.9	58.0	57.4	2863-2881 bp	1.0	68.9
ndhF_p4	GTCTTCGTTCCATGCCTCAT	AGAGCGGATGAATCAGAAACA	50.0	42.9	58.0	57.4	2757-2775 bp	1.0	68.8
ndhF_p5	CTGACCACATTCTCCATGGG	AGAGCGGATGAATCAGAAACA	55.0	42.9	58.0	57.4	2432-2443 bp	1.0	68.5