Hotspot

rpl32-trnL-UAG

SSC · 113491-114614 · 1124 bp

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Pi 0.0119
MarkerSeek score 57.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1124
variable_sites
25
pi
0.0119
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAAAAGGCTTTTTGTACGACA AGCGTGTCTACCGATTTCAC 38.1 50.0 55.6 58.0 1179-1242 bp 1.0 56.1
rpl32-trnL-UAG_p2 CAAAAGGCTTTTTGTACGACA CAGCGTGTCTACCGATTTCA 38.1 50.0 55.6 58.0 1180-1243 bp 1.0 56.1
rpl32-trnL-UAG_p3 CAAAAGGCTTTTTGTACGACA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 55.6 57.9 1189-1252 bp 1.0 55.1
rpl32-trnL-UAG_p4 CAAAAGGCTTTTTGTACGACA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 55.6 57.8 1188-1251 bp 1.0 54.6
rpl32-trnL-UAG_p5 CAAAAGGCTTTTTGTACGACA CTAAGAGCAGCGTGTCTACC 38.1 55.0 55.6 57.8 1187-1250 bp 1.0 54.6