Hotspot

rpl36-rps8

LSC · 80249-80748 · 500 bp

Back to Breynia

Pi 0.008

MarkerSeek score 58.90

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 8
pi: 0.008
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ATTCGCCCTCGTCTACGTAT	GGCGGGATGGGAATTGTAAT	50.0	50.0	58.4	58.0	673-684 bp	1.0	78.0
rpl36-rps8_p2	ATTCGCCCTCGTCTACGTAT	TAGGCGGGATGGGAATTGTA	50.0	50.0	58.4	58.2	675-686 bp	1.0	76.5
rpl36-rps8_p3	AATTCGCCCTCGTCTACGTA	GGCGGGATGGGAATTGTAAT	50.0	50.0	58.6	58.0	674-685 bp	1.0	76.2
rpl36-rps8_p4	TAATTCGCCCTCGTCTACGT	GGCGGGATGGGAATTGTAAT	50.0	50.0	58.6	58.0	675-686 bp	1.0	76.2
rpl36-rps8_p5	ATTCGCCCTCGTCTACGTAT	ATCTTAGGCGGGATGGGAAT	50.0	50.0	58.4	58.3	679-690 bp	1.0	76.0