Hotspot

ycf4-cemA

LSC · 61119-62196 · 1078 bp

Back to Breynia

Pi 0.0136

MarkerSeek score 57.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 1078
variable_sites: 29
pi: 0.0136
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GTCGCATCCTACTCCGATTC	CTTCCCTATCCACGATGCTG	55.0	55.0	57.9	57.8	1902-1932 bp	1.0	81.7
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	CTTCCCTATCCACGATGCTG	45.0	55.0	58.1	57.8	1726-1756 bp	1.0	81.6
ycf4-cemA_p3	TGGGGTTTTTGTTGGTTGGA	CTTCCCTATCCACGATGCTG	45.0	55.0	58.0	57.8	2145-2175 bp	1.0	81.4
ycf4-cemA_p4	GTCGCATCCTACTCCGATTC	ACTTCCCTATCCACGATGCT	55.0	50.0	57.9	58.2	1903-1933 bp	1.0	81.3
ycf4-cemA_p5	TGGGGTTTTTGTTGGTTGGA	ACTTCCCTATCCACGATGCT	45.0	50.0	58.0	58.2	2146-2176 bp	1.0	81.0