Hotspot

psaA-ycf3

LSC · 42708-43399 · 692 bp

Back to Breynia

Pi 0.0078

MarkerSeek score 58.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 10
pi: 0.0078
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	840-863 bp	1.0	79.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CGGAAGCTTGGTTCGATCAA	50.0	50.0	57.9	58.6	881-904 bp	1.0	77.5
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCTATAGCGCT	50.0	45.0	57.9	57.3	849-872 bp	1.0	76.5
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	GCTATAGCGCTTACTCCTGG	50.0	55.0	58.5	57.6	793-816 bp	1.0	76.5
psaA-ycf3_p5	TTGAGAAATGACCGGGTCTG	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.5	57.6	860-883 bp	1.0	76.3