Hotspot

atpA-atpF

LSC · 12160-12219 · 60 bp

Back to Breynia

Pi 0.0306

MarkerSeek score 58.40

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 3
pi: 0.0306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TACTAATCTCGTCGGCTCGA	ATTGGCATGTTTGGGACGAT	50.0	45.0	57.8	58.1	125-134 bp	1.0	81.9
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	ATTGGCATGTTTGGGACGAT	55.0	45.0	58.1	58.1	207-216 bp	1.0	81.1
atpA-atpF_p3	TAATCTCGTCGGCTCGAATG	ATTGGCATGTTTGGGACGAT	50.0	45.0	57.6	58.1	122-131 bp	1.0	80.6
atpA-atpF_p4	CCGTCGCCTACTTGAAGTAC	TATTGGCATGTTTGGGACGA	55.0	45.0	58.1	57.5	208-217 bp	1.0	78.3
atpA-atpF_p5	ACCGTCGCCTACTTGAAGTA	ATTGGCATGTTTGGGACGAT	50.0	45.0	58.5	58.1	208-217 bp	1.0	78.1