Hotspot

trnK-UUU-rps16

LSC · 4282-5067 · 786 bp

Back to Breynia

Pi 0.0083
MarkerSeek score 59.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
786
variable_sites
12
pi
0.0083
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGTCGCACTTAAAAGCCGA CCTTGAAAAAGGCGCTCAAC 45.0 50.0 58.1 58.2 910-1319 bp 1.0 82.0
trnK-UUU-rps16_p2 AAGTCGCACTTAAAAGCCGA CTTGAAAAAGGCGCTCAACC 45.0 50.0 58.1 58.2 909-1318 bp 1.0 82.0
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 897-1306 bp 1.0 81.8
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 897-1306 bp 1.0 81.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 896-1305 bp 1.0 81.8