Hotspot

ycf1

SSC · 125524-131031 · 5508 bp

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Pi 0.00771
MarkerSeek score 48.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5508
variable_sites
247
pi
0.00771
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GTTGTGTAGTGATTCTCGGACT ACGGTATTCCCAACAATCCG 45.5 50.0 57.8 58.0 1253-1286 bp 1.0 71.7
ycf1_p2 GTTGTGTAGTGATTCTCGGACT GTGCCCAAAGACGTAAGACA 45.5 50.0 57.8 58.1 1200-1233 bp 1.0 71.1
ycf1_p3 GTTGTGTAGTGATTCTCGGACT TCATCAAAGGTTCCATGCGT 45.5 45.0 57.8 57.8 1218-1251 bp 1.0 70.5
ycf1_p4 AGTTGTGTAGTGATTCTCGGAC ACGGTATTCCCAACAATCCG 45.5 50.0 57.8 58.0 1254-1287 bp 0.777778 62.0
ycf1_p5 AGTTGTGTAGTGATTCTCGGAC GTGCCCAAAGACGTAAGACA 45.5 50.0 57.8 58.1 1201-1234 bp 0.777778 61.5