Hotspot

rps8

LSC · 81259-81663 · 405 bp

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Pi 0.00851
MarkerSeek score 37.30
Primer available yes
Label rps8

Feature Metadata

label_name
rps8
feature_type
gene
region
LSC
length_bp
405
variable_sites
20
pi
0.00851
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8_p1 TAACGCCCTTTGGTATTCGG CTTAAACGCAACAACGGCAT 50.0 45.0 58.0 58.0 1471-1522 bp 1.0 86.2
rps8_p2 TAACGCCCTTTGGTATTCGG TGTCGCTGTGATTACGGATG 50.0 50.0 58.0 58.1 1559-1610 bp 1.0 85.7
rps8_p3 TTCGTCCTCTCCTACGGATC CTTAAACGCAACAACGGCAT 55.0 45.0 58.0 58.0 1217-1268 bp 0.962963 84.4
rps8_p4 TAACGCCCTTTGGTATTCGG CGATTGCCCGAGAATTGAGA 50.0 50.0 58.0 58.1 1392-1425 bp 0.925926 81.9
rps8_p5 TTCGTCCTCTCCTACGGATC CGATTGCCCGAGAATTGAGA 55.0 50.0 58.0 58.1 1139-1171 bp 0.888889 79.7