Hotspot

ndhF-rpl32

SSC · 114464-115368 · 905 bp

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Pi 0.0173
MarkerSeek score 71.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
905
variable_sites
77
pi
0.0173
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.96
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAAGTGGAATGAGAGGT TGAAAAAGCCTTCAATGCTGT 50.0 38.1 58.0 56.9 981-1073 bp 1.0 73.1
ndhF-rpl32_p2 TGGGAAGTGGAATGAGAGGT ATGAAAAAGCCTTCAATGCTGT 50.0 36.4 58.0 57.4 982-1074 bp 1.0 69.0
ndhF-rpl32_p3 TGGGAAGTGGAATGAGAGGT TCGCTAATGAAAAAGCCTTCA 50.0 38.1 58.0 56.2 988-1080 bp 1.0 67.2
ndhF-rpl32_p4 TGGGAAGTGGAATGAGAGGT TGAAAAAGCCTTCAATGCTG 50.0 40.0 58.0 55.1 981-1073 bp 1.0 66.3
ndhF-rpl32_p5 TGGGAAGTGGAATGAGAGGT GAAAAAGCCTTCAATGCTGT 50.0 40.0 58.0 55.0 980-1072 bp 1.0 66.3