Hotspot

psaJ-rpl33

LSC · 67947-68478 · 532 bp

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Pi 0.0112
MarkerSeek score 63.70
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
532
variable_sites
30
pi
0.0112
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 CTCTGGTTCGGGTCTTTAGC AACATCTTTTCCCTTGGCCA 55.0 45.0 58.0 57.6 431-652 bp 1.0 84.7
psaJ-rpl33_p2 TCCCAGATGCGTTGACATTT AACATCTTTTCCCTTGGCCA 45.0 45.0 57.8 57.6 382-603 bp 1.0 83.6
psaJ-rpl33_p3 TTCCCAGATGCGTTGACATT AACATCTTTTCCCTTGGCCA 45.0 45.0 57.8 57.6 383-604 bp 1.0 83.6
psaJ-rpl33_p4 AGTACTCTCTGGTTCGGGTC AACATCTTTTCCCTTGGCCA 55.0 45.0 58.2 57.6 437-658 bp 1.0 83.5
psaJ-rpl33_p5 GTACTCTCTGGTTCGGGTCT AACATCTTTTCCCTTGGCCA 55.0 45.0 58.2 57.6 436-657 bp 1.0 83.5