Hotspot

psbE-petL

LSC · 65343-66483 · 1141 bp

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Pi 0.00786
MarkerSeek score 67.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1141
variable_sites
63
pi
0.00786
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GCTGAGGTTATAGTTAAAGCTGC 50.0 43.5 57.8 57.6 729-1236 bp 1.0 59.7
psbE-petL_p2 ACGAATAACCAACCCGCAAT GCTGAGGTTATAGTTAAAGCTGC 45.0 43.5 58.2 57.6 815-1322 bp 1.0 59.3
psbE-petL_p3 GTTCTCCTGTGCTTCCAGAC GCTGAGGTTATAGTTAAAGCTGC 55.0 43.5 58.2 57.6 724-1231 bp 1.0 59.3
psbE-petL_p4 TGCTGACGAATAACCAACCC GCTGAGGTTATAGTTAAAGCTGC 50.0 43.5 58.2 57.6 820-1327 bp 1.0 59.1
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT GCTGAGGTTATAGTTAAAGCTGC 50.0 43.5 58.4 57.6 722-1229 bp 1.0 58.1