Hotspot

petA-psbJ

LSC · 63633-64535 · 903 bp

Back to Bredia

Pi 0.0114
MarkerSeek score 61.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
903
variable_sites
47
pi
0.0114
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGTTCTTCCTGGCATCTGTT ATACTCAGGGTTGGGCTCAT 45.0 50.0 57.0 58.1 887-1061 bp 1.0 79.3
petA-psbJ_p2 TTGTTCTTCCTGGCATCTGT ATACTCAGGGTTGGGCTCAT 45.0 50.0 57.0 58.1 888-1062 bp 1.0 79.3
petA-psbJ_p3 TGTTCTTCCTGGCATCTGTT GTTCATACTCAGGGTTGGGC 45.0 55.0 57.0 58.2 891-1065 bp 1.0 78.1
petA-psbJ_p4 TTGTTCTTCCTGGCATCTGT GTTCATACTCAGGGTTGGGC 45.0 55.0 57.0 58.2 892-1066 bp 1.0 78.1
petA-psbJ_p5 TGTTCTTCCTGGCATCTGTT TTCATACTCAGGGTTGGGCT 45.0 50.0 57.0 58.3 890-1064 bp 1.0 77.4