Hotspot

ndhC-trnV-UAC

LSC · 51390-52131 · 742 bp

Back to Bredia

Pi 0.017
MarkerSeek score 70.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
742
variable_sites
75
pi
0.017
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGACCTTTACTAATCGGGGC GGTTCGAATCCGTATAGCCC 55.0 55.0 57.7 57.9 776-907 bp 1.0 88.5
ndhC-trnV-UAC_p2 GGACCTTTACTAATCGGGGC CGGTTCGAATCCGTATAGCC 55.0 55.0 57.7 58.3 777-908 bp 1.0 86.6
ndhC-trnV-UAC_p3 GACCTTTACTAATCGGGGCG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.1 57.9 876 bp 0.037037 39.8
ndhC-trnV-UAC_p4 GACCTTTACTAATCGGGGCG CGGTTCGAATCCGTATAGCC 55.0 55.0 58.1 58.3 877 bp 0.037037 37.9
ndhC-trnV-UAC_p5 ACCTTTACTAATCGGGGCGA GGTTCGAATCCGTATAGCCC 50.0 55.0 58.5 57.9 875 bp 0.037037 36.8