Hotspot

ndhK-ndhC

LSC · 50973-51026 · 54 bp

Back to Bredia

Pi 0.0428
MarkerSeek score 66.70
Primer available yes
Label ndhK-ndhC

Feature Metadata

label_name
ndhK-ndhC
feature_type
igs
region
LSC
length_bp
54
variable_sites
7
pi
0.0428
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK-ndhC_p1 TTTGGGTTGTTCGAGCAAGT CATGGCGAAAAGGAGCATTG 45.0 50.0 58.2 58.1 127-135 bp 1.0 84.8
ndhK-ndhC_p2 TGGGTTGTTCGAGCAAGTAA CATGGCGAAAAGGAGCATTG 45.0 50.0 57.4 58.1 125-133 bp 1.0 81.8
ndhK-ndhC_p3 TTGGGTTGTTCGAGCAAGTA CATGGCGAAAAGGAGCATTG 45.0 50.0 57.4 58.1 126-134 bp 1.0 81.8
ndhK-ndhC_p4 TTTTGGGTTGTTCGAGCAAG CATGGCGAAAAGGAGCATTG 45.0 50.0 57.1 58.1 128-136 bp 1.0 79.8
ndhK-ndhC_p5 TGGGTTGTTCGAGCAAGTAAA CATGGCGAAAAGGAGCATTG 42.9 50.0 58.1 58.1 125-133 bp 1.0 78.2