Hotspot

psbM-trnD-GUC

LSC · 29948-30676 · 729 bp

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Pi 0.00838
MarkerSeek score 63.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
729
variable_sites
36
pi
0.00838
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCACCTTGACTCACGGTTTT TCAATTGGTTAGAGCACCGC 45.0 50.0 57.3 58.6 877-907 bp 1.0 75.4
psbM-trnD-GUC_p2 AATCACCTTGACTCACGGTT TCAATTGGTTAGAGCACCGC 45.0 50.0 57.1 58.6 879-909 bp 1.0 73.6
psbM-trnD-GUC_p3 ATCACCTTGACTCACGGTTT TCAATTGGTTAGAGCACCGC 45.0 50.0 57.1 58.6 878-908 bp 1.0 73.6
psbM-trnD-GUC_p4 TCACCTTGACTCACGGTTTTT TCAATTGGTTAGAGCACCGC 42.9 50.0 58.0 58.6 877-907 bp 1.0 73.0
psbM-trnD-GUC_p5 ATCACCTTGACTCACGGTTTT TCAATTGGTTAGAGCACCGC 42.9 50.0 57.8 58.6 878-908 bp 1.0 71.4