Hotspot

trnS-GCU-trnR-UCU

LSC · 7935-9738 · 1804 bp

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Pi 0.00978
MarkerSeek score 62.50
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1804
variable_sites
89
pi
0.00978
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 1866-1917 bp 1.0 52.7
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 1865-1916 bp 1.0 52.7
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AGAAGACCTATGTCCTATCCA 50.0 42.9 58.0 54.6 1860-1911 bp 1.0 52.5
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AGAAGACCTATGTCCTATCCA 50.0 42.9 58.0 54.6 1859-1910 bp 1.0 52.5
trnS-GCU-trnR-UCU_p5 CAATCCGACGCTTTAGTCCA ACCAAAGGTTTAGAAGACCTATGT 50.0 37.5 58.0 57.6 1871-1922 bp 1.0 52.1