Hotspot

trnH-GUG-psbA

LSC · 76-306 · 231 bp

Back to Bredia

Pi 0.0533
MarkerSeek score 90.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
231
variable_sites
46
pi
0.0533
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.97
markerseek_score
90.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 342-437 bp 1.0 90.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 356-451 bp 1.0 90.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 355-450 bp 1.0 90.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 357-452 bp 1.0 90.4
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 292-387 bp 0.962963 89.0