Hotspot

ndhE-ndhG

SSC · 117132-117375 · 244 bp

Back to Braya

Pi 0.0268
MarkerSeek score 59.70
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
244
variable_sites
14
pi
0.0268
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 GGCACTCAAAACAAGTACATGT AATTTCGGTGGCTCGTCAAT 40.9 45.0 57.4 58.2 265-318 bp 1.0 65.9
ndhE-ndhG_p2 AGGCACTCAAAACAAGTACATG AATTTCGGTGGCTCGTCAAT 40.9 45.0 57.2 58.2 266-319 bp 1.0 63.7
ndhE-ndhG_p3 GGCACTCAAAACAAGTACATGT TTTCGGTGGCTCGTCAATAA 40.9 45.0 57.4 57.5 263-316 bp 1.0 63.6
ndhE-ndhG_p4 AGGCACTCAAAACAAGTACA AATTTCGGTGGCTCGTCAAT 40.0 45.0 55.1 58.2 266-319 bp 1.0 63.3
ndhE-ndhG_p5 GGCACTCAAAACAAGTACATGTT AATTTCGGTGGCTCGTCAAT 39.1 45.0 58.1 58.2 265-318 bp 1.0 61.9