Hotspot

ccsA-ndhD

SSC · 114471-114683 · 213 bp

Back to Braya

Pi 0.0235
MarkerSeek score 59.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
213
variable_sites
11
pi
0.0235
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCTGCAATTGTGGCTTCT GCAAATGACAAGGTCGAATCC 40.0 47.6 55.9 57.9 364-388 bp 1.0 59.1
ccsA-ndhD_p2 TGGATATGCTATTTTGGCGTCA GCAAATGACAAGGTCGAATCC 40.9 47.6 58.2 57.9 326-350 bp 1.0 58.9
ccsA-ndhD_p3 TGCTATTTTGGCGTCAATCT GCAAATGACAAGGTCGAATCC 40.0 47.6 55.7 57.9 320-344 bp 1.0 58.4
ccsA-ndhD_p4 AATTCTGCAATTGTGGCTTCT GCAAATGACAAGGTCGAATCC 38.1 47.6 56.7 57.9 365-389 bp 1.0 57.3
ccsA-ndhD_p5 TGCTATTTTGGCGTCAATCTT GCAAATGACAAGGTCGAATCC 38.1 47.6 56.5 57.9 320-344 bp 1.0 56.6