Hotspot

rpl32-trnL-CUA

SSC · 112835-113295 · 461 bp

Back to Braya

Pi 0.0242
MarkerSeek score 60.70
Primer available yes
Label rpl32-trnL-CUA

Feature Metadata

label_name
rpl32-trnL-CUA
feature_type
igs
region
SSC
length_bp
461
variable_sites
25
pi
0.0242
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CUA_p1 CTTTCTACAGGTAATTCAAAAAGT AGCAGCGTGTCTACCAATTT 29.2 45.0 53.5 57.8 541-903 bp 1.0 44.7
rpl32-trnL-CUA_p2 CTTTCTACAGGTAATTCAAAAAGT GAGCAGCGTGTCTACCAATT 29.2 50.0 53.5 58.3 542-904 bp 1.0 44.7
rpl32-trnL-CUA_p3 CTTTCTACAGGTAATTCAAAAAGT TCCTAAGAGCAGCGTGTCTA 29.2 50.0 53.5 57.9 548-910 bp 1.0 44.6
rpl32-trnL-CUA_p4 CTTTCTACAGGTAATTCAAAAAGT CCTAAGAGCAGCGTGTCTAC 29.2 55.0 53.5 57.8 547-909 bp 1.0 44.6
rpl32-trnL-CUA_p5 CTTTCTACAGGTAATTCAAAAAGT CTAAGAGCAGCGTGTCTACC 29.2 55.0 53.5 57.8 546-908 bp 1.0 44.6