Pi
0.0337
MarkerSeek score
69.60
Primer available
yes
Label
ndhF-rpl32
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhF-rpl32_p1 | GTGGAATGAAAGGGATGATCCA | ACGAATACGCTTTTTCGAGA | 45.5 | 40.0 | 58.1 | 55.5 | 669-964 bp | 1.0 | 53.5 |
| ndhF-rpl32_p2 | AGTGGAATGAAAGGGATGATCC | ACGAATACGCTTTTTCGAGA | 45.5 | 40.0 | 57.8 | 55.5 | 670-965 bp | 1.0 | 52.9 |
| ndhF-rpl32_p3 | TGGAATGAAAGGGATGATCCA | ACGAATACGCTTTTTCGAGA | 42.9 | 40.0 | 56.7 | 55.5 | 668-963 bp | 1.0 | 51.6 |
| ndhF-rpl32_p4 | GTGGAATGAAAGGGATGATCCA | ACGAATACGCTTTTTCGAGAT | 45.5 | 38.1 | 58.1 | 56.1 | 669-964 bp | 1.0 | 50.0 |
| ndhF-rpl32_p5 | AGTGGAATGAAAGGGATGATCC | ACGAATACGCTTTTTCGAGAT | 45.5 | 38.1 | 57.8 | 56.1 | 670-965 bp | 1.0 | 49.9 |