Hotspot

petD-rpoA

LSC · 76811-76996 · 186 bp

Back to Braya

Pi 0.0337
MarkerSeek score 59.60
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
186
variable_sites
13
pi
0.0337
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TGGTTAGGTATTGGAGCAACA ATTGAACATTTTCACATAGAAGA 42.9 26.1 56.9 52.4 306-309 bp 1.0 43.4
petD-rpoA_p2 TGGTTAGGTATTGGAGCAACA TTGAACATTTTCACATAGAAGAT 42.9 26.1 56.9 52.4 305-308 bp 1.0 43.4
petD-rpoA_p3 TGGTTAGGTATTGGAGCAACA AATTGAACATTTTCACATAGAAGA 42.9 25.0 56.9 53.2 307-310 bp 1.0 43.4
petD-rpoA_p4 TGGTTAGGTATTGGAGCAACA ATTGAACATTTTCACATAGAAGAT 42.9 25.0 56.9 53.0 306-309 bp 1.0 43.4
petD-rpoA_p5 AGGTATTGGAGCAACATTACCA TTGAACATTTTCACATAGAAGAT 40.9 26.1 57.4 52.4 300-303 bp 1.0 43.4