Hotspot

psaJ-rpl33

LSC · 66134-66540 · 407 bp

Back to Braya

Pi 0.0309
MarkerSeek score 60.50
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
407
variable_sites
27
pi
0.0309
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACATATCTTTCCGTAGCGCC AACATCTTTACCCTTGGCCA 50.0 45.0 57.8 57.0 542-548 bp 1.0 75.4
psaJ-rpl33_p2 TAGCGCCGGTACTAAGTACT AACATCTTTACCCTTGGCCA 50.0 45.0 57.7 57.0 529-535 bp 1.0 74.1
psaJ-rpl33_p3 ACATATCTTTCCGTAGCGCC GAACATCTTTACCCTTGGCCA 50.0 47.6 57.8 58.2 543-549 bp 1.0 73.8
psaJ-rpl33_p4 ACATATCTTTCCGTAGCGCC CGAACATCTTTACCCTTGGC 50.0 50.0 57.8 56.8 544-550 bp 1.0 73.7
psaJ-rpl33_p5 ACATATCTTTCCGTAGCGCC ACATCTTTACCCTTGGCCAT 50.0 45.0 57.8 56.8 541-547 bp 1.0 73.5