Hotspot

ycf4-cemA

LSC · 59649-60101 · 453 bp

Back to Braya

Pi 0.0146
MarkerSeek score 64.50
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
453
variable_sites
16
pi
0.0146
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCTTACTTCTTGCGTGT AGAGAGATCAACCAGGGCAA 45.0 50.0 58.0 58.3 531-566 bp 1.0 83.3
ycf4-cemA_p2 TTGGCTTACTTCTTGCGTGT TCAACCAGGGCAAAAAGACT 45.0 45.0 58.0 57.5 524-559 bp 1.0 82.4
ycf4-cemA_p3 TTGGCTTACTTCTTGCGTGT GAGAGATCAACCAGGGCAAA 45.0 50.0 58.0 57.5 530-565 bp 1.0 82.1
ycf4-cemA_p4 TTGGCTTACTTCTTGCGTGT AGAGAGAGATCAACCAGGGC 45.0 55.0 58.0 58.5 533-568 bp 1.0 82.0
ycf4-cemA_p5 TTGGCTTACTTCTTGCGTGT GCAGAGAGAGATCAACCAGG 45.0 55.0 58.0 57.4 535-570 bp 1.0 81.2