Hotspot

ndhK-ndhC

LSC · 49258-49320 · 63 bp

Pi 0.0513

MarkerSeek score 64.50

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 7
pi: 0.0513
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GGCTGGAAAGTCTTGACCAA	GGCGAAAGGGAGCATTAGAA	50.0	50.0	58.0	58.0	187-196 bp	1.0	84.6
ndhK-ndhC_p2	GTTGTTCGATCAAGTACGGGA	GGCGAAAGGGAGCATTAGAA	47.6	50.0	58.0	58.0	125-134 bp	1.0	78.7
ndhK-ndhC_p3	AGGCTGGAAAGTCTTGACCA	GGCGAAAGGGAGCATTAGAA	50.0	50.0	58.9	58.0	188-197 bp	1.0	77.8
ndhK-ndhC_p4	ACCATAAAGAAGCGGCCATA	GGCGAAAGGGAGCATTAGAA	45.0	50.0	57.0	58.0	207-216 bp	1.0	76.1
ndhK-ndhC_p5	GGCTGGAAAGTCTTGACCAA	TGGCGAAAGGGAGCATTAGA	50.0	50.0	58.0	59.1	188-197 bp	1.0	76.1