Hotspot

psbM-trnD-GAC

LSC · 28584-29546 · 963 bp

Back to Braya

Pi 0.0125
MarkerSeek score 58.70
Primer available yes
Label psbM-trnD-GAC

Feature Metadata

label_name
psbM-trnD-GAC
feature_type
igs
region
LSC
length_bp
963
variable_sites
30
pi
0.0125
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GAC_p1 TGAGAATGAAGAGTGCAGTAGC GTTCGAGCCCCGTCAGTC 45.5 66.7 58.1 60.1 1033-1043 bp 1.0 43.3
psbM-trnD-GAC_p2 GAGAATGAAGAGTGCAGTAGC GTTCGAGCCCCGTCAGTC 47.6 66.7 56.4 60.1 1032-1042 bp 1.0 43.3
psbM-trnD-GAC_p3 AGGAATGAGAATGAAGAGTGCA GTTCGAGCCCCGTCAGTC 40.9 66.7 57.4 60.1 1038-1048 bp 1.0 43.3
psbM-trnD-GAC_p4 TGAGAATGAAGAGTGCAGTAGC GAGCACCGCCCTGTCAAG 45.5 66.7 58.1 60.7 1064-1074 bp 1.0 43.2
psbM-trnD-GAC_p5 TGAGAATGAAGAGTGCAGTAGC GCCCTGTCAAGGCGGAAG 45.5 66.7 58.1 60.7 1057-1067 bp 1.0 43.2