Hotspot

trnG-GGA-trnR-AGA

LSC · 9096-9253 · 158 bp

Back to Braya

Pi 0.0266
MarkerSeek score 59.50
Primer available yes
Label trnG-GGA-trnR-AGA

Feature Metadata

label_name
trnG-GGA-trnR-AGA
feature_type
igs
region
LSC
length_bp
158
variable_sites
11
pi
0.0266
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GGA-trnR-AGA_p1 AGCCTTCCAAGCTAACGATG TGATATTGCGTCCAATAGGA 50.0 40.0 58.0 53.9 279-284 bp 1.0 51.1
trnG-GGA-trnR-AGA_p2 ACCCTTAGCCTTCCAAGCTA TGATATTGCGTCCAATAGGA 50.0 40.0 58.0 53.9 285-290 bp 1.0 51.0
trnG-GGA-trnR-AGA_p3 CGACGTCGACTAAAACCCTT TGATATTGCGTCCAATAGGA 50.0 40.0 57.9 53.9 299-304 bp 1.0 50.7
trnG-GGA-trnR-AGA_p4 AAACCCTTAGCCTTCCAAGC TGATATTGCGTCCAATAGGA 50.0 40.0 58.1 53.9 287-292 bp 1.0 50.7
trnG-GGA-trnR-AGA_p5 AGCCTTCCAAGCTAACGATG TTGATATTGCGTCCAATAGGA 50.0 38.1 58.0 54.8 280-285 bp 1.0 50.1