Hotspot

trnS-AGC-trnG-GGA

LSC · 7650-8301 · 652 bp

Back to Braya

Pi 0.0214
MarkerSeek score 61.10
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
652
variable_sites
32
pi
0.0214
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 CCAACGCTTTAGTCCACTCA ACGAATCACACTTTTACCACT 50.0 38.1 57.8 55.5 700-737 bp 1.0 57.4
trnS-AGC-trnG-GGA_p2 AACGCTTTAGTCCACTCAGC ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 698-735 bp 1.0 57.1
trnS-AGC-trnG-GGA_p3 CCAACGCTTTAGTCCACTCA AACGAATCACACTTTTACCACT 50.0 36.4 57.8 56.2 701-738 bp 1.0 55.5
trnS-AGC-trnG-GGA_p4 GCTTTAGTCCACTCAGCCAT ACGAATCACACTTTTACCACT 50.0 38.1 57.6 55.5 695-732 bp 1.0 55.5
trnS-AGC-trnG-GGA_p5 AACGCTTTAGTCCACTCAGC AACGAATCACACTTTTACCACT 50.0 36.4 58.2 56.2 699-736 bp 1.0 55.2