Hotspot

psbA

LSC · 259-1320 · 1062 bp

Back to Brassica

Pi 0.00298

MarkerSeek score 28.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 12
pi: 0.00298
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CTGCCTTAATCCACTTGGCT	CTTTACGGGGGAAGACCAAG	50.0	55.0	57.9	57.9	2020-2086 bp	1.0	82.8
psbA_p2	ACTGCCTTAATCCACTTGGC	CTTTACGGGGGAAGACCAAG	50.0	55.0	58.2	57.9	2021-2087 bp	1.0	82.6
psbA_p3	CTGCCTTAATCCACTTGGCT	TGCGGGTTTGGTATTTGGAT	50.0	45.0	57.9	57.8	1946-2012 bp	1.0	82.0
psbA_p4	ACTGCCTTAATCCACTTGGC	TGCGGGTTTGGTATTTGGAT	50.0	45.0	58.2	57.8	1947-2013 bp	1.0	81.8
psbA_p5	CTGCCTTAATCCACTTGGCT	TCTTTACGGGGGAAGACCAA	50.0	50.0	57.9	58.3	2021-2087 bp	1.0	81.5